Tumor antigen peptide is a new class of tumor markers. Tumor antigens are decomposed in the cell to generate peptides, which bind to MHC-1 to form MHC-1/antigen peptide complexes, and are displayed on cancer cell surface. The complexes can be recognized by corresponding CD8 T cells, and therefore can be used as targets for diagnosis and treatment. Identification of cancer cell tumor antigen peptides is, therefore, of great importance. We have developed a technical platform that integrates immune-biochemical / biophysical-bioinformatics methods to identify tumor antigen peptides. Our studies on cultured cells have shown that the identified antigenic peptides can induce a highly specific tumor killing effect.